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Electrophoresis is the technique used for seperation of NUCLEIC acids . The technique in brief involves making the gel based on the molecule being seprated , like agarose gel etc . Buffer prepration , making wells in gel while gel is setting with help of combs , adding ethidium bromide to gel for flouroscence development . Load the samples in the well and connect to power pack . Gel runs lighter bands move faster and heavier bands move slow . Control or marker is loaded in one if the wells for comparision and lastly gel is observed under illuminator .

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